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Description:
The role of the choroid plexus (CP) in brain homeostasis is being increasingly recognized and recent studies suggest that the CP has a more important role in physiological and pathological brain functions than currently appreciated. To obtain additional insight on the CP function, we performed a proteomics and transcriptomics characterization employing a combination of high resolution tandem mass spectrometry and gene expression analyses in normal rodent brain. Using multiple protein fractionation approaches, we identified 1400 CP proteins in adult CP. Microarray-based comparison of CP gene expression with the kidney, cortex and hippocampus showed significant overlap between the CP and the kidney. CP gene profiles were validated by in situ hybridization analysis of several target genes including klotho, CLIC 6, OATP 14 and Ezrin. Immunohistochemical analyses were performed for CP and enpendyma detection of several target proteins including cytokeratin, Rab7, klotho, tissue inhibitor of metalloprotease 1 (TIMP1), MMP9 and glial fibrillary acidic protein (GFAP). The molecular functions associated with various proteins of the CP proteome indicate that it is a blood-cerebrospinal fluid (CSF) barrier that exhibits high levels of metabolic activity. We also analyzed the gene expression changes induced by stress, an exacerbating factor for many illnesses, particularly mood disorders. Chronic stress altered the expression of several genes, downregulating 5HT2C, glucocorticoid receptor and the cilia genes IFT88 and smoothened while upregulating 5HT2A, BDNF, TNFα and IL-1b. The data presented here attach additional significance to the emerging importance of CP function in brain health and CNS disease states.
Sample Summary:
Rat choroid plexus samples were analyzedafter fractionation by 1) reverse-phase chromatography (fractionated by a) equal volume, or b) peak detection), 2) SDS-PAGE, 3) 2-dimensional LC (chromotofocusing, followed by reverse phase LC). See supplemental file for summary of datasets.
Publications:
Sathyanesan M, Girgenti MJ, Banasr M, Stone K, Bruce C, Guilchicek E, Wilczak-Havill K, Nairn A, Williams K, Sass S, Duman JG, Newton SS. (2012) Transl Psychiatry. 2012 Jul 10;2:e139. doi: 10.1038/tp.2012.64. A molecular characterization of the choroid plexus and stress-induced gene regulation. PubMed
Acknowledgement: This work was supported by funding from the National Institute of Health (MH 072894), Neuroproteomics Center grant (5P30DA018343), NARSAD Young Investigator award to Mounira Banasr, the Connecticut Mental Health Center and the Department of Mental Health and Addiction Services.
Supplemental File: Sathyanesan choroid plexus 2012.xlsx
Blib File: Choroid_Plexus_2012.blib
282 results available, displaying 1 to 10.[First/Prev] 1, 2, 3, 4, 5, 6, 7, 8 [Next/Last]
Analysis Type Sample Name Search Engine Database Date User/PI
LCMS CP FPLC 1 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:38:56.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 10 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:37:20.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 11 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:36:14.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 13 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:34:29.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 14 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:33:05.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 16 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:32:06.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 17 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:30:41.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 18 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:29:06.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 2 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:28:14.0 Samuel Sathyanesan/Samuel Sathyanesan
LCMS CP FPLC 21 info resources MASCOT IPI_rat_20080120.fasta 2008-12-27 16:27:15.0 Samuel Sathyanesan/Samuel Sathyanesan
DownLoad Peptide Summary pep_score > pep_homology
DownLoad Peptide Summary (all peptides with protein ids)


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Updated 21 Mar 2014 9:03