|Lys-C cleavage peptides from the immunoprecipitated soluble fragment of the cytosolic domain (sf-CD) of γ-secretase-cleaved peptidylglycine α-amidating monooxygenase (PAM). Titanium dioxide column-bound (phosphopeptide enriched) or -flow-through (phosphopeptide depleted) peptides were analyzed by MALDI-MS.|
|See summary file "Eipper 2010 sample descriptions.xlsx"|
|Rajagopal, C., Stone, K., Mains, R., and Eipper, B. (2010) Secretion Stimulates Intramembrane Proteolysis of a Secretory Granule Membrane Enzyme. J. Biol. Chem. 285: 34632-42.
Acknowledgement: This work was supported, in whole or in part, by National Institutes of Health Grant DK32949 (NIDDK) and National Institute on Drug Abuse Neuroproteomics Center Grant P30 DA018343. Instrumentation was obtained with funds from National Institutes of Health National Center for Research Resources Clinical and Translational Science Award UL1 RR024139 and Shared Instrumentation Grant 1S10RR024617.
|Analysis Type||Sample Name||Search Engine||Database||Date||User/PI|
|LCMS||sf-CD EN include list info resources||MASCOT||IPI_rat_20091220.fasta||2010-02-18 15:53:36.0||Chitra Rajagopal/Betty Eipper|
|LCMS||sf-CD FT include list info resources||MASCOT||IPI_rat_20091220.fasta||2010-02-18 15:48:33.0||Chitra Rajagopal/Betty Eipper|
|LCMS||soluable CD Swiss prot database info resources||MASCOT||SwissProt_55.6.fasta||2008-08-26 09:45:28.0||Betty Eipper/Betty Eipper|
|DownLoad Peptide Summary pep_score > pep_homology|
|DownLoad Peptide Summary (all peptides with protein ids)|